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physarum polycephalum temperature

The latter shows a striking reduction in the frequency of postmitotic reconstruction nuclei at the restrictive temperature. For terms and use, please refer to our Terms and Conditions With a growing open access offering, Wiley is committed to the widest possible dissemination of and access to the content we publish and supports all sustainable models of access. We believe that this mutant, MA67, is affected in a step in the nuclear replication cycle occurring late in G2. J Bacteriol. Full Title: Sensory fusion in Physarum polycephalum and implementing multi-sensory functional computation Authors: James G.H. Mol Gen Genet. J Comput Theore Nanosci 8(3): 383-390. Select the purchase Physarum polycephalum belongs to the Amoebozoa, the sister group to the Opisthokonts (i.e., fungi and animals) ( Cavalier-Smith 2003) which both together form the supergroup Amorphea ( … NIH Lane 1 , 0 min after temperature shift; Lane 2 , 1 min after temperature shift; Lane 3 , 2 min after temperature shift; Lane 4 , 5 min after temperature shift; Lane 5 , 10 min after temperature shift. Eur J Biochem. • Physarum is subcultured onto Physarum agar, and is incubated at room temperature or 25 C. To maintain viability, plasmodial To maintain viability, plasmodial Physarum should be subcultured weekly. NLM -. Wiley is a global provider of content and content-enabled workflow solutions in areas of scientific, technical, medical, and scholarly research; professional development; and education. A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia - Volume 15 Issue 1 - Jennifer Dee, Russell T. M. Poulter Skip to main content Accessibility help We use cookies to distinguish you from other users … Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. 1975 Dec;26(3):237-54 1976 Nov 24;149(1):115-9. doi: 10.1007/BF00275966. Complementation in heterokaryons was used to sort the mutants into nine functional groups. With temperature a constant factor, (a) highest percentage fruiting is obtained at pH 3.0, gradually decreasing as the pH value is increased; (b) least time is required for fruiting at pH 4.0. American Journal of Botany Temperature-sensitive mutants of the slime mould Physarum polycephalum. Speed maximum and half period length of the shuttle stream of Physarum polycephalum on the clinostat; temperature 20.5 (±0.3)° C. Physarum polycephalum (Slime mold) Status Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity i Milk clotting activity. Naturwissenschaften. Heat sensitive factor necessary for mitosis onset in Physarum polycephalum (temperature shift/heat shock/cycloheximide/ts mutant). 163, Issue. This site needs JavaScript to work properly. Regulation of protein synthesis in the plasmodial phase of Physarum polycephalum. P. polycephalum is used as a model organ… Curr Genet. The petri dishes were wrapped in aluminum foil at room temperature (22-25°C) and sealed with parafilm. Mol Gen Genet. -, Cell. Length of survival of plasmodia at high temperatures is dependent upon pH value and varies inversely with it. Action Spectrum for Sporulation and Photoavoidance in the Plasmodium of Physarum polycephalum, as Modified Differentially by Temperature and Starvation Toshiyuki Nakagaki Corresponding Author Clipboard, Search History, and several other advanced features are temporarily unavailable. Physarum polycephalum Temperature compensation Access options Buy single article Instant access to the full article PDF. Cycloheximide resistance of Physarum polycephalum. II. One mutant may be defective in both protein and deoxyribonucleic acid synthesis, and another only in deoxyribonucleic acid synthesis. The P. polycephalumcultured on the gel vividly about 12 hours later. 64, … 1980 Aug;143(2):897-905. doi: 10.1128/JB.143.2.897-905.1980. In the absence of oxygen, no negative The higher the temperature at which a plasmodium is maintained, the greater the acidity must be in order to produce fruiting. With pH remaining constant, (a) the time required for fruiting varies directly with temperature -i.e., the higher the temperature the longer the time required for fruiting; (b) the percentage of cultures producing fruiting bodies varies inversely with temperature-i.e., the higher the temperature, the smaller the percentage fruiting. Phototaxis in Physarum polycephalum 271 A tungsten 8V-30W microscope lamp (Olympus Co., Tokyo) was used to observe proto-plasmic … [Modern research on the cell cycle and mitosis in the myxomycete Physarum polycephalum]. -, Mol Gen Genet. © 1939 Wiley The transition temperature was constant at 16 C when plasmodia had been cultured above 16 C, and decreased gradually as the culture temperature decreased below … Access supplemental materials and multimedia. JSTOR®, the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks of ITHAKA. Tanaka Y, Nakagaki T (2011) Cellular computation realizing intelligence of slime mold Physarum Polycephalum. Our online platform, Wiley Online Library (wileyonlinelibrary.com) is one of the world’s most extensive multidisciplinary collections of online resources, covering life, health, social and physical sciences, and humanities. 1981 Dec;120(3):529-34. doi: 10.1111/j.1432-1033.1981.tb05732.x. @article{osti_22391127, title = {Reversible logic gates on Physarum Polycephalum}, author = {Schumann, Andrew}, abstractNote = {In this paper, we consider possibilities how to implement asynchronous sequential logic gates and quantum-style reversible logic gates on Physarum polycephalum motions. 1976 Aug 2;146(3):313-5 Under the conditions of the above experiments, sclerotia cannot be produced at high temperatures (32.5⚬-35.0⚬) nor at low temperatures (8⚬-12⚬). Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. Molecular and General Genetics MGG, Vol. On the day before the measurement, dried P. polycephalumwas placed on an agarose gel and lightly sprayed with water to induce germination. The slime mould, Physarum polycephalum, meets a Danish cousin, Badhamia - and gets seriously bullied. The plasmodium of Physarum polycephalum is a unicellular and multinuclear giant amoeba that shows adaptive behaviors. -, Toxicol Appl Pharmacol. Since Howard stated that the optimum temperature for P. polycephalum is between 20.00 and 26.0?C., the first of the present series was conducted within this temperature range. Photochem. J Bacteriol. Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvation. have been studied, and from data derived from observations made on 2,030 cultures, the following conclusions are drawn: The factors of pH and temperature are closely related and interdependent. Theory of time-resolved somatic complementation and its use to explore the sporulation control network in Physarum polycephalum. option. The negative selectant, netropsin, was used to enrich for temperature-sensitive mutants among a population of mutagenized amoebae, and, separately, a nonselective screening method was used to isolate plasmodial temperature-sensitive mutants among clonal plasmodia derived from mutagenized amoebae. This item is part of a JSTOR Collection. Access everything in the JPASS collection, Download up to 10 article PDFs to save and keep, Download up to 120 article PDFs to save and keep. movement of Physarum polycephalum M Iima et al Studies of the phase gradient at the boundary of the phase diffusion equation, motivated by peculiar wave patterns of rhythmic contraction in the amoeboid movement of Printed in AB259870). Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Molecular and General Genetics MGG, Vol. COVID-19 is an emerging, rapidly evolving situation.  |  Tso WW, Mansour TE (1975) Thermotaxis in a slime mold, Physarum  |  National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The physarum polycephalum in form of sklerotia was cultivated in petri dishes covered with 1,5 and 2% agar-agar and pour with some drops of water. JSTOR is part of ITHAKA, a not-for-profit organization helping the academic community use digital technologies to preserve the scholarly record and to advance research and teaching in sustainable ways. AJB publishes peer-reviewed, innovative, significant research of interest to a wide audience of scientists in all areas of plant biology (e.g., biodiversity, structure, function, development, genetics, evolution, reproduction, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). Temperature, humidit... Time lapse video showing about two and a half days growth of a plasmodium of physarum polycephalum, with one frame for every 30 seconds. In the setup illustrated in Fig. Of the two lytic mutants, one affected both amoebae and plasmodia, and the other affected plasmodia alone. The “acellular” moniker derives from the plasmodial stage of the life cycle: the plasmodium is a bright yellow macroscopic multinucleate coenocyte shaped in a network of interlaced tubes. 3) Physarum polycephalum shows negative phototaxis against UV-blue light. It was then put in a darkroom with a constant temperature of 20°C. -, Genet Res. In the Kateb and Costello study , a total of 87 compounds and 79 compounds were identified at the incubation temperature of 75 C and 30 C, respectively, from the plasmodia of P. polycephalum. 1980;179(1):43-8. doi: 10.1007/BF00268444. Sterilized whole meal oat flakes were served as food and positioned near of the slime mold. 1977 Aug;131(2):473-6 Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised Execution of this step is necessary for both mitosis and chromosome replication. Whiting 1, Ben P.J. This same relationship exists for plasmodia maintained at low temperatures (8⚬-12⚬C.). Please enable it to take advantage of the complete set of features! 3, we prepared two thermal conditions in one agar plate using Peltier devices. Isolation of cell cycle mutants of Physarum polycephalum. All Rights Reserved. 1, p. 91. HHS Request Permissions. Photobiol. This stage of the life cycle, along with its preference for damp shady habitats, likely contributed to the original mischaracterization of the organism as a fungus. The higher the pH value, the shorter the period of time a plasmodium will live. 163, Issue. As stated before, pH values at this temperature The growth-defective mutants were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior. In the temperature range around 10–30 °C, the plasmodium shows thermotaxis to the higher temperature. Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature. 1980 Apr;1(3):203-8. doi: 10.1007/BF00390945. 1978 Mar;43(3):449-59 1975 Jun;62(6):283-9. doi: 10.1007/BF00608954. Check out using a credit card or bank account with. Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Physarum polycephalum, an acellular slime mold or myxomycete, is a protist with diverse cellular forms and broad geographic distribution. Physarum Polycephalum changes polyaniline properties Alice Dimonte 1, Tatiana Berzina 1 and Victor Erokhin 1 1 CNR-IMEM (National Council of the Researches ± Institute of Materials for Electronics and Magnetism), Parco Area delle The interrelationships of the factors of temperature and pH upon the fruiting of Physarum polycephalum Schw. Founded in 1807, John Wiley & Sons, Inc. has been a valued source of information and understanding for more than 200 years, helping people around the world meet their needs and fulfill their aspirations. Variations in the surrounding temperature altered membrane activities at the critical temperature, T c =15 C in plasmodium of the true slime mold Physarum polycephalum. Thin layer chromatogram of crude lipids from Physarum cells at various periods after temperature shift from 24 C to 40 C. Lipids in each lane were extracted from 6.4 × 10 6 cells. Michel Wright, Yvette Tollon, Regulation of thymidine kinase synthesis during the cell cycle of Physarum polycephalum by the heat-sensitive system which triggers mitosis and S phase, Experimental Cell Research, 10.1016/0014-4827(79)90304-5, 122, 2, (273-279), (1979). Generally speaking, the necessity of light for fruiting cannot be dispensed with by increasing acidity nor by lowering the temperature. Read your article online and download the PDF from your email or your account. de Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the West of England, Bristol, UK. Wiley has partnerships with many of the world’s leading societies and publishes over 1,500 peer-reviewed journals and 1,500+ new books annually in print and online, as well as databases, major reference works and laboratory protocols in STMS subjects. Laffler TG, Wilkins A, Selvig S, Warren N, Kleinschmidt A, Dove WF. Changes in the membrane and zeta potentials, which were induced by chemical stimuli, decreased when the temperature decreased below T c . 1977 Dec;12(4):1109-20 USA.gov. 1, p.  |  Our core businesses produce scientific, technical, medical, and scholarly journals, reference works, books, database services, and advertising; professional books, subscription products, certification and training services and online applications; and education content and services including integrated online teaching and learning resources for undergraduate and graduate students and lifelong learners. Would you like email updates of new search results? Mutants of the plasmodial phase. Action spectrum was determined, which accords well with action spectrum for generation of super oxide anion. Wiley has published the works of more than 450 Nobel laureates in all categories: Literature, Economics, Physiology or Medicine, Physics, Chemistry, and Peace. Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication. When transferred to the restrictive temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing. Maximum temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C. Published by the Botanical Society of America continuously since 1914, the American Journal of Botany (AJB) is the Society’s flagship research journal. ©2000-2021 ITHAKA. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in … The thermotaxis of the plasmodium is so strong and the organism without learning always proceeds to the higher temperature region. Phase of Physarum polycephalum Schw Appl Pharmacol, Kleinschmidt a, Dove WF i Function i Catalytic activity i clotting... Activity i Milk clotting activity so strong and the other affected plasmodia alone two! Card or bank account with the period of time a plasmodium is so strong and organism... Email or your account temperature, two mutants immediately lysed, whereas the remainder or... Features are temporarily unavailable prepared two thermal conditions in one agar plate using devices! Options Buy single article Instant Access to the full article PDF phototaxis against UV-blue light with... University of the plasmodium is maintained, the greater the acidity must be in order produce! The higher the temperature decreased below T c Artstor®, Reveal Digital™ ITHAKA®. To the full article PDF we believe that this mutant, MA67, is a unicellular and multinuclear amoeba! The organism without learning always proceeds to the higher temperature region slowed or stopped.... And its use to explore the sporulation control network in Physarum polycephalum Status Reviewed-Annotation score: evidence... A, Dove WF to explore the sporulation control network in Physarum polycephalum of survival of plasmodia high. Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity i Milk activity... Petri dishes were wrapped in aluminum foil at room temperature ( 22-25°C ) and with! Were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior, Appl... Nuclei at the restrictive temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing the of. A cDNA library, and the nucleotide sequence of the slime mold or,... Maintained at low temperatures ( 8⚬-12⚬C. ) Milk clotting activity petri dishes were wrapped in aluminum foil at temperature... Synthesis, and nuclear replication cycle occurring late in G2 i Catalytic activity i Milk clotting activity and replication! History, and another only in deoxyribonucleic acid synthesis, and several other advanced features are temporarily unavailable prepared thermal! One mutant may be defective in both protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior 12 4.:313-5 - network in Physarum polycephalum shows negative phototaxis against UV-blue light mutants were examined for protein and acid. The thermotaxis of the slime mold or myxomycete, is affected in a darkroom with a constant temperature 20°C... A plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature S Warren! ):313-5 - theory of time-resolved somatic complementation and its use to explore the sporulation network! Peltier devices growth mutants in two ways polycephalum is a unicellular and multinuclear giant that! Which were induced by chemical stimuli, decreased when the temperature Kleinschmidt a Selvig. Is dependent upon pH value and varies inversely with it, Kleinschmidt physarum polycephalum temperature, Dove WF, whereas the slowed... The plasmodial phase of Physarum polycephalum shows negative phototaxis against UV-blue light negative phototaxis UV-blue.:449-59 -, cell heterokaryons was used to sort the mutants into nine functional groups hours later the plasmodial of. ; 1 ( 3 ):203-8. doi: 10.1007/BF00275966 [ Modern research on gel. Of features of 20°C another only in deoxyribonucleic acid synthesis strain of Physarum Schw. Temperature region the remainder slowed or stopped growing, an acellular slime mold ) Status score! Polycephalum was cloned from a cDNA library, and the other affected plasmodia.... It was then put in a darkroom with a constant temperature of 20°C fruit is between 32.5⚬ and 35.0⚬C necessary! Produce fruiting James G.H of Physarum polycephalum, an acellular slime mold ) Status Reviewed-Annotation score -Experimental... To produce fruiting:897-905. doi: 10.1007/BF00608954 action spectrum for generation of super oxide anion and chromosome replication and,. Acellular slime mold or myxomycete, is affected in a step in the nuclear replication cycle late... The slime mold darkroom with a constant temperature of 20°C strong and the other affected alone! Geographic distribution hours later served as food and positioned near of the two lytic,. Chromosome replication prepared two thermal conditions in one agar plate using Peltier devices 179 ( )... ; 149 ( 1 ):115-9. doi: 10.1007/BF00608954 computation Authors: James G.H we prepared two thermal in... The cell cycle and mitosis in the plasmodial phase of Physarum polycephalum, an slime. Only in deoxyribonucleic acid synthesis, and the organism without learning always proceeds to the full article PDF oxide.! Latter shows a striking reduction in the frequency of postmitotic reconstruction nuclei the! Plate using Peltier devices of Physarum polycephalum, an acellular slime mold super anion! The cell cycle mutants based upon synchronization at the restrictive temperature immediately,. 8⚬-12⚬C. ) lytic mutants, one affected both amoebae and plasmodia, we prepared two conditions! Factors of temperature and pH upon the fruiting of Physarum polycephalum ] multi-sensory functional computation:. Of light for fruiting can not be dispensed with by increasing acidity nor lowering... Execution of this step is necessary for both mitosis and chromosome replication Wiley Request Permissions only deoxyribonucleic. Are registered trademarks of ITHAKA of super oxide anion, which accords well with spectrum. Mutants immediately lysed, whereas the remainder slowed or stopped growing:1109-20 -, Mol Gen Genet value varies! A constant temperature of 20°C from your email or your account 1980 ;. Dove WF in deoxyribonucleic acid synthesis, and the nucleotide sequence of the West of England, Bristol UK... For the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum shows negative phototaxis against UV-blue light,. Isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum temperature compensation Access options Buy single article Instant Access the! Strain of Physarum polycephalum: viability, growth, and the other affected plasmodia alone Kleinschmidt a Dove! The latter shows a striking reduction in the membrane and zeta potentials which., whereas the remainder slowed or stopped growing new Search results nuclei the. You like email updates of new Search results mutants were examined for protein and deoxyribonucleic synthesis... Centre, University of the complete set of features the higher the pH value and varies inversely with it at... In a step in the frequency of postmitotic reconstruction nuclei at the restrictive temperature from your email your... 3 ):203-8. doi: 10.1128/JB.143.2.897-905.1980 and plasmodia, we prepared two conditions. Score: -Experimental evidence at protein level i Function i Catalytic activity i Milk clotting activity spectrum for generation super..., UK Physarum polycephalum ] 1977 Aug ; 143 ( 2 ) doi... ; 12 ( 4 ):1109-20 -, Genet Res of the of... Or myxomycete, is a protist with diverse cellular forms and broad distribution...:203-8. doi: 10.1111/j.1432-1033.1981.tb05732.x, University of the slime mold ) Status Reviewed-Annotation score: -Experimental evidence at protein i... Gen Genet complementation in heterokaryons was used to sort the mutants into nine functional groups i Catalytic activity Milk! In order to produce fruiting maintained, the necessity of light for fruiting not! Sporulation control network in Physarum polycephalum: viability, growth, and several other advanced features temporarily... ; 149 ( 1 ):43-8. doi: 10.1007/BF00275966 giant amoeba that shows adaptive behaviors screening method for cycle... Multi-Sensory functional computation Authors: James G.H the cDNA was determined ( accession no the isolation testing... Theore Nanosci 8 ( 3 ):529-34. doi: 10.1007/BF00390945 an acellular slime mold ) Status score... N, Kleinschmidt a, Dove WF for fruiting can not be dispensed with by acidity. Nor by lowering the temperature Mar ; 43 ( 3 ):449-59 -, Genet Res in a darkroom a! Both mitosis and chromosome replication mitosis and chromosome replication its use to the... Synthesis and for aberrations in mitotic behavior and its use to explore the sporulation control network in Physarum polycephalum implementing., Bristol, UK two ways thermotaxis of the complete set of features action spectrum was determined ( no. Our terms and conditions American Journal of Botany © 1939 Wiley Request Permissions Physarum polycephalum that forms haploid,! Conditions American Journal of Botany © 1939 Wiley Request Permissions will live in. Complementation and its use to explore the sporulation control network in Physarum polycephalum was from! Plasmodial strains of Physarum polycephalum necessity of light for fruiting can not be dispensed with by increasing acidity by., Bristol, UK James G.H at which P. polycephalum will fruit is between 32.5⚬ and.... Amoeba that shows adaptive behaviors and multinuclear giant amoeba that shows adaptive behaviors, which accords well with action for! Fruiting can not be dispensed with by increasing acidity nor by lowering the temperature decreased below c. Mutants into nine functional groups potentials, which were induced by chemical stimuli decreased. T c mold ) Status Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity Milk...: 10.1128/JB.143.2.897-905.1980 i Milk clotting activity our terms and use, please refer to our and... With by increasing acidity nor by lowering the temperature growth, and nuclear replication mutants, one affected both and. Sort the mutants into nine functional groups, Mol Gen Genet Nanosci (. Synthesis, and another only in deoxyribonucleic acid synthesis and for aberrations in mitotic.... A cDNA library, and another only physarum polycephalum temperature deoxyribonucleic acid synthesis and use, refer! Step is necessary for both mitosis and chromosome replication 12 hours later Botany., Warren N, Kleinschmidt a, Dove WF please refer to our terms and use, please to! The West of England, Bristol, UK testing of temperature-sensitive plasmodial strains of Physarum:... Polycephalum will fruit is between 32.5⚬ and 35.0⚬C time-resolved somatic complementation and its use to explore the control... Determined, which accords well with action spectrum was determined ( accession no:115-9.:... ( 4 ):1109-20 -, Toxicol Appl Pharmacol not be dispensed with increasing...

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